BBT301 Techniques in Biotechnology Syllabus
AKTU B.Tech · 3rd Semester · Unit-wise syllabus (effective 2023-24)
BBT301 Techniques in Biotechnology (also written as KBT301) is a subject in Semester 3 of the AKTU B.Tech programme, prescribed by Dr. A.P.J. Abdul Kalam Technical University. It carries 4 credits with an L-T-P of 3-1-0. The syllabus is divided into 5 units. The complete unit-wise topic list, course outcomes and reference books are given below, taken from the official AKTU syllabus effective from 2023-24.
BBT301 Subject Details
| Subject Code | BBT301 / KBT301 |
|---|---|
| Subject Name | Techniques in Biotechnology |
| University | Dr. A.P.J. Abdul Kalam Technical University (AKTU) |
| Course | B.Tech (Bachelor of Technology) |
| Year / Semester | 2nd Year · 3rd Semester |
| Credits | 4 |
| L-T-P | 3-1-0 |
| Total Units | 5 |
| Scheme Effective From | 2023-24 |
| Last Verified | 2026-08-15 |
BBT301 Techniques in Biotechnology Unit-Wise Syllabus
Official AKTU syllabus (effective 2023-24) — 5 units.
Unit 1
Light microscopy, Bright & Dark Field microscopy, Fluorescence microscopy, Phase Contrast microscopy, Electron microscopy: TEM and SEM, Atomic force microscopy and con focal scanning laser microscopy. Differential interference contrast microscopy.
Unit 2
Principle and Operations of Chromatography, Thin layer chromatography, Ion Exchange Chromatography, High Performance Liquid Chromatography (HPLC), Gas Liquid Chromatography (GLC), Gel Filtration Chromatography, Affinity Chromatography.
Unit 3
Electromagnetic radiation and spectrum,Atomic absorption and Atomic emission spectroscopy,Principle, working and applications of UV-VIS, NMR, ESR and IR spectrometer, Principle and applications of Mass Spectroscopy, Circular Dichorism (CD) principles, Principle and applications of Positron Emission Tomography(PET), Basics of X-Ray diffraction analysis and their application in biotechnology .
Unit 4
Theory of Electrophoresis, Factors affecting the migration of substances Gel electrophoresis, PAGE, SDS-PAGE, Agarose Electrophoresis of Nucleic Acid, Isoelectric Focusing of Protein Pulse Gel Electrophoresis and Western Blotting. Theory of centrifugation and sedimentation. Types of centrifuges, Preparative and analytical centrifugation; Density gradient centrifugation. Application of centrifugation for preparative and analytical purpose.
Unit 5
Principles of 3-D printing, 3-D Bioprinting of tissues, organs and bacteria.Ideal material properties for bioprinting, Biosensors: Principles and definition, characteristics of Ideal biosensors, Biochemical components of biosensors, Bioaffinity systems, Immunosensors. Principle and working of Flow Cytometry and cell sorter.
BBT301 Course Outcomes
- At the end of course , the student will be able to
- CO 1 Acquire knowledge on types of microscope and its applications in Biotechnology. K2
- CO 2 Apply the principle of chromatographic techniques for qualitative and quantitative K3
- analysis of biomolecules.
- CO 3 Employ various spectroscopic techniques for qualitative and quantitative analysis of Bio- K3
- Molecules/Bio-Analytes.
- CO 4 Employ various electrophoresis and centrifugation techniques for analysis of Bio- K3
- Molecules/Bio-Analytes.
- CO 5 Acquire knowledge on 3 D printing, flow cytometry and biosensors. K2
BBT301 Reference Books
- Wilson, K, Walker, J., Principles and Techniques of Practical Biochemistry. 5th Ed. - Cambridge University Press,. Cambridge
- Sabari Ghosal&Anupama Sharma Awasthi., Fundamentals of Bioanalytical Techniques and Instrumentation, PHI learning
- Bioanalytical Techniques by A. Shourie and S SChapadgaonkar. TERI Press. 2015
- Immunoassay and Other Bioanalytical Techniques.Jeanette M. van Emon. CRC press. 2006
- 3D Bioprinting in Regenerative Engineering: Principles and Applications, Ali Khademhosseini&Gulden Camci-Unal, CRC
Frequently Asked Questions about BBT301
What is the syllabus of BBT301 Techniques in Biotechnology?
The AKTU BBT301 Techniques in Biotechnology syllabus is divided into 5 units. The complete topic list for each unit is published on this page, taken from the official Dr. A.P.J. Abdul Kalam Technical University syllabus effective from 2023-24.
How many units are there in BBT301?
BBT301 Techniques in Biotechnology has 5 units in the AKTU syllabus, with an L-T-P (Lecture-Tutorial-Practical) structure of 3-1-0 and 4 credits.
Is BBT301 the same subject as KBT301?
Yes. AKTU renumbered its B.Tech subject codes from the 2022-23 session onwards, so the same subject appears as BBT301 and KBT301 depending on the scheme year. The syllabus content is the same.
Which semester is BBT301 taught in?
BBT301 Techniques in Biotechnology is taught in Semester 3 of the 2nd year of the AKTU B.Tech programme.
How many credits is BBT301 worth?
BBT301 Techniques in Biotechnology carries 4 credits in the AKTU evaluation scheme, with an L-T-P of 3-1-0.
Where can I download BBT301 previous year question papers?
AKTU B.Tech previous year question papers for every semester are available to download free on Ryzenstudy, with no login or registration required.
Is this the official AKTU syllabus for BBT301?
Yes. Every topic on this page is taken from the official Dr. A.P.J. Abdul Kalam Technical University syllabus document (AKTU B.Tech 2nd Year Biotechnology Syllabus), effective from 2023-24. A direct link to the original AKTU PDF is provided at the top of this page. Last verified on 2026-08-15.
Related AKTU Syllabus
Source: Dr. A.P.J. Abdul Kalam Technical University official syllabus — AKTU B.Tech 2nd Year Biotechnology Syllabus, effective from 2023-24. Ryzenstudy is not affiliated with AKTU. Content last verified on 2026-08-15.