BBT403 Enzyme Engineering Syllabus
AKTU B.Tech · 4th Semester · Unit-wise syllabus (effective 2023-24)
BBT403 Enzyme Engineering (also written as KBT403) is a subject in Semester 4 of the AKTU B.Tech programme, prescribed by Dr. A.P.J. Abdul Kalam Technical University. It carries 3 credits with an L-T-P of 3-0-0. The syllabus is divided into 5 units: Introduction to enzymes, Enzyme inhibition, Enzyme Immobilization, Enzyme Biosensors. The complete unit-wise topic list, course outcomes and reference books are given below, taken from the official AKTU syllabus effective from 2023-24.
BBT403 Subject Details
| Subject Code | BBT403 / KBT403 |
|---|---|
| Subject Name | Enzyme Engineering |
| University | Dr. A.P.J. Abdul Kalam Technical University (AKTU) |
| Course | B.Tech (Bachelor of Technology) |
| Year / Semester | 2nd Year · 4th Semester |
| Credits | 3 |
| L-T-P | 3-0-0 |
| Total Units | 5 |
| Scheme Effective From | 2023-24 |
| Last Verified | 2026-08-15 |
BBT403 Enzyme Engineering Unit-Wise Syllabus
Official AKTU syllabus (effective 2023-24) — 5 units.
Unit 1: Introduction to enzymes
Introduction to enzymes: Holoenzyme, apoenzyme, prosthetic group. Interaction between enzyme and substratelock and key model, induced fit model. Features of active site, activation energy, enzyme specificity and types. IUB system of classification and nomenclature of enzymes. Kinetics of single substrate reactions; Derivation of Michaelis - Menten equation, turnover number; determination of Km and Vmax (LB plot, ED plot), Importance of Km &Vmax; Numerical related to enzyme kinetics, Multi-Substrate reaction mechanisms.
Unit 2: Enzyme inhibition
Factors affecting the velocity of enzyme catalyzed reaction- enzyme concentration, temperature, pH, substrate concentration, inhibitors and activators. Enzyme inhibition: irreversible; reversible (competitive, uncompetitive and non competitive inhibition); Substrate and Product inhibition, Allosteric regulation of enzymes, concerted & sequential model; Deactivation Kinetics.
Unit 3
Extraction of crude enzyme from plant, animal and microbial source; some case study. Purification of enzymes by the help of different methods. Methods of characterization of enzymes; criteria of purity. Unit of enzyme activity - definition and importance. Development of enzyme assays.
Unit 4: Enzyme Immobilization
Enzyme Immobilization: Adsorption, Matrix entrapment, Encapsulation, Cross linking, Covalent binding and their examples; Advantages and disadvantages of different immobilization techniques. Structure & stability of immobilized enzymes, kinetic properties of immobilized enzymes- partition effect, diffusion effect. Overview of applications of immobilized enzyme systems.
Unit 5: Enzyme Biosensors
Enzyme Biosensors: elements of biosensors, three generations of biosensors, Types of biosensors: calorimetric, potentiometric, amperometric, optical and piezoelectric. Design of enzyme electrodes and their applications as biosensors in industry, health care and environment. Design of Immobilized Enzyme Reactors- Stirred tank reactors(STR), Continuous Flow Stirred Tank Reactors (CSTR), Packed- bed reactors (PBR), Fluidized-bed Reactors (FBR); Membrane reactors.
BBT403 Course Outcomes
- At the end of course , the student will be able to
- CO 1 Understand the concepts of enzyme and their kinetics. K2
- CO 2 Explain the factors affecting enzymatic reactions and role of inhibitors. K3
- CO 3 Perform the extraction, purification & characterization of different enzyme from K3
- different source.
- CO 4 Understand the concept of enzyme immobilization and its applications. K4
- CO 5 Apply knowledge of enzyme in developing biosensors and bioreactors. K3
BBT403 Reference Books
- Fundamentals of enzymology by Nicolas C. price and Lewis stevens. Oxford University Press
- Enzymes by Trevor palmer, East west Press
- Enzyme Technology by Messing.
- Enzymes: Dixon and Webb. (IRL Press)
- Enzyme technology by Chaplin and Bucke. Cambridge Univerity Press
- Biochemical engineering fundamentals, second edition. James E Bailey, David F., Ollis, McGraw Hill Intl. Edition
Frequently Asked Questions about BBT403
What is the syllabus of BBT403 Enzyme Engineering?
The AKTU BBT403 Enzyme Engineering syllabus is divided into 5 units: Introduction to enzymes; Enzyme inhibition; Enzyme Immobilization; Enzyme Biosensors. The complete topic list for each unit is published on this page, taken from the official Dr. A.P.J. Abdul Kalam Technical University syllabus effective from 2023-24.
How many units are there in BBT403?
BBT403 Enzyme Engineering has 5 units in the AKTU syllabus, with an L-T-P (Lecture-Tutorial-Practical) structure of 3-0-0 and 3 credits.
Is BBT403 the same subject as KBT403?
Yes. AKTU renumbered its B.Tech subject codes from the 2022-23 session onwards, so the same subject appears as BBT403 and KBT403 depending on the scheme year. The syllabus content is the same.
Which semester is BBT403 taught in?
BBT403 Enzyme Engineering is taught in Semester 4 of the 2nd year of the AKTU B.Tech programme.
How many credits is BBT403 worth?
BBT403 Enzyme Engineering carries 3 credits in the AKTU evaluation scheme, with an L-T-P of 3-0-0.
Where can I download BBT403 previous year question papers?
AKTU B.Tech previous year question papers for every semester are available to download free on Ryzenstudy, with no login or registration required.
Is this the official AKTU syllabus for BBT403?
Yes. Every topic on this page is taken from the official Dr. A.P.J. Abdul Kalam Technical University syllabus document (AKTU B.Tech 2nd Year Biotechnology Syllabus), effective from 2023-24. A direct link to the original AKTU PDF is provided at the top of this page. Last verified on 2026-08-15.
Related AKTU Syllabus
Source: Dr. A.P.J. Abdul Kalam Technical University official syllabus — AKTU B.Tech 2nd Year Biotechnology Syllabus, effective from 2023-24. Ryzenstudy is not affiliated with AKTU. Content last verified on 2026-08-15.